Staphylococcus aureus rezistence pret antimikrobajām vielām un meticilīnrezistences gēna mecA noteikšana

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RESEARCH PRODUCT

Staphylococcus aureus rezistence pret antimikrobajām vielām un meticilīnrezistences gēna mecA noteikšana

subject

Staphylococcus aureus antibakteriālā rezistence Farmācija MRSA mecA

description

Staphylococcus aureus ir viens no galvenajiem patogēniem, kas spēj izraisīt ādas un mīksto audu infekcijas. Rezistence pret antibiotikām ir saistīta ar plašo virulences faktoru klāstu un tā spēju izmantot horizontālu gēnu pārnesi, it īpaši meticilīnrezistences gēna mecA pārnesi. MRSA celmi vēl joprojām ir izplatīta problēma veselības aprūpē. Darba mērķis: Noteikt Staphylococcus aureus klīnisko izolātu jutību pret antimikrobiālajiem līdzekļiem un mecA gēna klātesamību, izmantojot BBL™ disku difūzijas testu, E-testu un FilmArray ® Multiplex PCR metodi. Rezultāti: Visaugstākā antibakteriālā jutība bija pret rifampicīnu (98,91%), kā arī augsta jutība bija pret cefoksitīnu (96,74%) un trimetoprimu/sulfametoksazolu (95,10%). Visi 368 S.aureus celmi uzrādīja 100% jutību pret vankomicīnu. Analizējot ar Multiplex PCR 11 S.aureus izolātus, 8 tika apstiprināti (72,73%) kā MRSA. Atslēgvārdi: Staphylococcus aureus, MRSA, mecA, antibakteriālā rezistence

year	journal	country	edition	language
2022-01-01

https://dspace.lu.lv/dspace/handle/7/59778

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