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RESEARCH PRODUCT
Human conglutinin-like protein inhibits infection by the human immunodeficiency virus-1 in vitro.
H. UshijimaHeinz-christoph SchröderS. PoznanovicE. MatthesW. E. G. Müllersubject
ImmunologyHIV Envelope Protein gp120Antiviral AgentsVirusConglutininViral envelopeVirologyLectinsHumansBinding sitechemistry.chemical_classificationAcquired Immunodeficiency SyndromebiologyBinding proteinComplement Fixation TestsLectinVirologyMolecular biologyIn vitroCollectinsMannose-Binding Lectinschemistrybiology.proteinHIV-1Serum GlobulinsGlycoproteinCarrier ProteinsMannoseProtein Bindingdescription
In summary the lectin-like protein analogous to bovine conglutinin was purified from human serum. Using a lectin-based ELISA system, it was demonstrated that conglutinin-like protein binds to human immunodeficiency virus-1 (HIV1) glycoprotein 120 (gp 120) via its carbohydrate binding site. In vitro experiments with T-lymphoblastoid CEM cells revealed that conglutinin-like protein abolishes infection by HIV1; a 50 % cytoprotective concentration of 23.9 μg/ml was measured.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1992-03-01 | Research in virology |