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RESEARCH PRODUCT
Listeria monocytogenes Differential Transcriptome Analysis Reveals Temperature-Dependent Agr Regulation and Suggests Overlaps with Other Regulons
Anne-laure VivantAnne-laure VivantPascal PiveteauPascal PiveteauDominique GarmynYoann AugagneurYoann AugagneurLaurent Galsubject
MicroarraysOperonMutantmedicine.disease_causeTranscriptomesTranscriptomeMolecular Cell BiologyTranscriptional regulationCluster AnalysisAmino AcidsCellular Stress ResponsesGeneticsRegulation of gene expression0303 health sciencesMultidisciplinaryQRTemperatureSalt ToleranceGenomicsPlanktonFunctional GenomicsBacterial Pathogens[SDV.MP]Life Sciences [q-bio]/Microbiology and ParasitologyMedicineResearch Articleagr-alisteria monocytogenes;pathogenic organism;transcriptome;temperature;agr-aScienceSigma FactorBiologyRegulonMicrobiologyMicrobial Ecology03 medical and health sciencesListeria monocytogenes[ SDV.SA.AGRO ] Life Sciences [q-bio]/Agricultural sciences/AgronomyGenome Analysis ToolsmedicinePathogenic organismGene SilencingBiology030304 developmental biologyGram Positive[ SDV ] Life Sciences [q-bio]030306 microbiologyGene Expression ProfilingComputational BiologyBiological TransportGene Expression Regulation BacterialListeria monocytogenesGene expression profilingRegulonBiofilmsTranscriptomelisteria monocytogènesGene DeletionTranscription Factorsdescription
Listeria monocytogenes is a ubiquitous, opportunistic pathogenic organism. Environmental adaptation requires constant regulation of gene expression. Among transcriptional regulators, AgrA is part of an auto-induction system. Temperature is an environmental cue critical for in vivo adaptation. In order to investigate how temperature may affect AgrA-dependent transcription, we compared the transcriptomes of the parental strain L. monocytogenes EGD-e and its Delta agrA mutant at the saprophytic temperature of 25 degrees C and in vivo temperature of 37 degrees C. Variations of transcriptome were higher at 37 degrees C than at 25 degrees C. Results suggested that AgrA may be involved in the regulation of nitrogen transport, amino acids, purine and pyrimidine biosynthetic pathways and phage-related functions. Deregulations resulted in a growth advantage at 37 degrees C, but affected salt tolerance. Finally, our results suggest overlaps with PrfA, sigma B, sigma H and CodY regulons. These overlaps may suggest that through AgrA, Listeria monocytogenes integrates information on its biotic environment
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2012-09-14 |