Amplified fragment length polymorphism (AFLP) and biochemical typing of Photobacterium damselae subsp. damselae.

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RESEARCH PRODUCT

Amplified fragment length polymorphism (AFLP) and biochemical typing of Photobacterium damselae subsp. damselae.

S. Botella S. Botella María J. Pujalte María Antonia Ferrús Javier Hernández M.c. Macián Esperanza Garay

subject

Genetics DNA Bacterial Photobacterium Dendrogram UPGMA General Medicine Phenotypic trait Aquaculture Biology Subspecies Photobacterium biology.organism_classification Applied Microbiology and Biotechnology Microbiology Bacterial Typing Techniques Perciformes Fish Diseases Photobacterium damselae Phenotype Animals Amplified fragment length polymorphism Typing Gram-Negative Bacterial Infections Polymorphism Restriction Fragment Length Biotechnology

description

Aims: The aim of the present study was to characterize subspecifically Photobacterium damselae subsp. damselae strains isolated from cultured Sparus aurata and Dicentrarchus labrax by means of phenotypic and molecular typing techniques (amplified fragment length polymorphism, AFLP). Methods and Results: Seventy-one strains of P. damselae subsp. damselae were isolated from 38 cultured fishes at different fish farms located on the Mediterranean coast near Valencia, Spain. Most fish studied were asymptomatic and some were recovered during infectious outbreaks. Phenotypic characterization revealed a considerable degree of variability within the subspecies, including some characters, such as production of urease, which are used to differentiate P. damselae subsp. damselae from P. damselae subsp. piscicida. Genetic characterization was conducted on a selection of 33 strains, including two reference strains. Dice coefficient (Sd) and the unweighted pair group method with average linkage (UPGMA) were used for numerical analysis of banding patterns. AFLP type was defined on the basis of 100% similarity in the dendrogram obtained, yielding 24 distinct AFLP profiles. At 70% similarity, 13 clusters were defined, thus confirming the great variability observed for the phenotypic traits. Conclusions: The AFLP variability shown by the isolates was high enough to discriminate between different strains which colonize the same fish. However, closely related AFLP types were usually derived from strains isolated at the same fish farm, indicating an epidemiological relationship. Significance and Impact of the Study: This study has confirmed that the AFLP technique allows discrimination of individual strains within P. damselae subsp. damselae for epidemiological studies, and that this subspecies exhibits greater variability than that described for subspecies piscicida.

year	journal	country	edition	language
2002-09-18	Journal of applied microbiology

10.1046/j.1365-2672.2002.01748.x https://pubmed.ncbi.nlm.nih.gov/12234352