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RESEARCH PRODUCT

Seeding nerve sutures with minced nerve-graft (MINE-G): a simple method to improve nerve regeneration in rats

Roberto PuleioGiovanni CassataLuca CiceroAnna MartoranaFulvio PlesciaFrancesco MoschellaAlessio Vincenzo MarioloAdriana CordovaGrazia SalimbeniGiovanni ZabbiaSalvatore D'arpaCarla Cannizzaro

subject

0301 basic medicinemedicine.medical_specialtyrat sciatic nerveTransplantation AutologousRats sprague dawleyNeurosurgical ProceduresRats Sprague-Dawley03 medical and health sciencesRandom Allocation0302 clinical medicinePeripheral Nerve InjuriesRisk FactorsImage Interpretation Computer-AssistedMedicineAnimalsnerve regenerationRandom allocationNerve reconstructionAnalysis of Variancebusiness.industryRegeneration (biology)Graft SurvivalSuture TechniquesNerve graftGeneral MedicineAnatomySciatic NerveSurgeryRatsDisease Models Animal030104 developmental biologyTissue TransplantationNerve graftTissue and Organ HarvestingSeedingSurgerySciatic nervenerve reconstructionbusinessNerve suture030217 neurology & neurosurgery

description

Background: The aim of this study was to assess the effect of seeding the distal nerve suture with nerve fragments in rats. Methods: On 20 rats, a 15 mm sciatic nerve defect was reconstructed with a nerve autograft. In the Study Group (10 rats), a minced 1 mm nerve segment was seeded around the nerve suture. In the Control Group (10 rats), a nerve graft alone was used. At 4 and 12 weeks, a walking track analysis with open field test (WTA), hystomorphometry (number of myelinated fibers (n), fiber density (FD) and fiber area (FA) and soleus and gastrocnemius muscle weight ratios (MWR) were evaluated. The Student t-test was used for statistical analysis. Results: At 4 and 12 weeks the Study Group had a significantly higher n and FD (p =.043 and.033). The SMWR was significantly higher in the Study Group at 12 weeks (p =.0207). Conclusions: Seeding the distal nerve suture with nerve fragments increases the number of myelinated fibers, the FD and the SMWR. The technique seems promising and deserves further investigation to clarify the mechanisms involved and its functional effects.

yearjournalcountryeditionlanguage
2017-07-26
10.1080/00015458.2017.1353237http://hdl.handle.net/10447/276461