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RESEARCH PRODUCT
Application of high-content screening for the study of hepatotoxicity: Focus on food toxicology
M. Teresa DonatoLaia Tolosasubject
0303 health sciencesComputer scienceFood toxicologyCellular imagingEnergy metabolismFood Contamination04 agricultural and veterinary sciencesGeneral MedicineAutomated microscopyToxicology040401 food scienceCell LineAutomation03 medical and health sciences0404 agricultural biotechnologyDrug developmentHigh-content screeningImage Processing Computer-AssistedAnimalsHumansBiochemical engineeringChemical and Drug Induced Liver InjuryCells Cultured030304 developmental biologyFood Sciencedescription
Safety evaluation of thousands of chemicals that are directly added to or come in contact with food is needed. Due to the central role of the liver in intermediary and energy metabolism and in the biotransformation of foreign compounds, the hepatotoxicity assessment is essential. New approach methodologies have been proposed for the safety evaluation of compounds with the idea of rapidly gaining insight into effects on biochemical mechanisms and cellular processes and screening large number of compounds. In this sense, high-content screening (HCS) is the application of automated microscopy and image analysis for better understanding of complex biological functions and mechanisms of toxicity. HCS multiparametric measurements have been shown to be a useful tool in early toxicity testing during drug development, but also in assessing the impact from food chemicals and environmental toxicants. Reviewing the use of cellular imaging technology in the safety evaluation of food-relevant chemicals offers evidence about the impact of this technology in safety assessment.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2020-06-20 | Food and Chemical Toxicology |