6533b860fe1ef96bd12c2e90
RESEARCH PRODUCT
Development and characterization of 11 microsatellite markers in the rock sparrow, Petronia petronia
Alessandro GrapputoMirko De GirolamoFederica BarbisanLorenzo ZaneAndrea Pilastrosubject
GeneticsZ chromosomeEcologybiologyLocus (genetics)biology.organism_classificationBiochemistryGeneral Biochemistry Genetics and Molecular BiologyLoss of heterozygosityRock sparrowPetroniaMicrosatelliteAmplified fragment length polymorphismSex linkagedescription
Eleven polymorphic microsatellite loci were isolated in the passeriform Petronia petronia using the enrichment protocol FIASCO (fast isolation by AFLP of sequences containing repeats). We detected three to 13 alleles per locus in 25 specimens collected from an Italian population. The level of expected heterozygosity ranged from 0.439 to 0.856. One locus is sex linked to the Z chromosome. The total exclusionary probabilities using these loci for the first and the second parent were 0.978 and 0.999, respectively. These are the first microsatellite loci characterized from the rock sparrow that can be used for estimating population structure and for large-scale parentage analysis.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2006-11-21 | Molecular Ecology Notes |