6533b860fe1ef96bd12c319a

RESEARCH PRODUCT

Analysis of Hemolymph Ecdysteroids in the Female Earwig: Labidura riparia

Jean-paul DelbecqueColete StrambiMichel VancasselAlain StrambiMaryvonne Foraste

subject

0106 biological sciencesmedicine.medical_specialtyLabiduridaeecdysteroidsmedicine.medical_treatmentmedia_common.quotation_subject[SDV]Life Sciences [q-bio]Ovaryreproductive cycle regulation010603 evolutionary biology01 natural sciences03 medical and health scienceschemistry.chemical_compoundInternal medicineHemolymphmedicine030304 developmental biologymedia_common0303 health sciencesbiologyLabidura ripariabiology.organism_classificationSteroid hormoneEndocrinologymedicine.anatomical_structurechemistryEarwigearwigAnimal Science and ZoologyReproductionEcdysoneDevelopmental Biology

description

International audience; The nature of hemolymph ecdysteroids was analyzed by high performance liquid chromatography-radioimmunoassay, during the two main phases of the reproductive cycles of Labidura riparia. 20-hydroxyecdysone and ecdysone were present together with polar and apoiar products.Changes in the ecdysone/20-hydroxyecdysone ratio were evidenced according to the ovarian development. During natural (feeding and sexual phase of the cycle) or induced ovarian development, an increase of the ratio occurred whereas, during ovarian rest (fasting and/or parental phase), a significant decrease was observed.Up to 40 days after ovariectomy, immunoreactive compounds were observed in the hemolymph which corresponded partly to 20-hydroxyecdysone, ecdysone, and apoiar products. However, a large increase was observed in the proportions of the polar products which were mainly resolved, on the action of Helix enzymes, into 20-hydroxyecdysone and ecdysone.

10.1080/07924259.1991.9672175https://univ-rennes.hal.science/hal-01365333