6533b860fe1ef96bd12c3229
RESEARCH PRODUCT
A Xenogeneic-Free Protocol for Isolation and Expansion of Human Adipose Stem Cells for Clinical Uses
Angel Ayuso-sacidoCarolina GandíaAndres Sanz-garciaIsabel Pascual MorenoFrancisco J. EstebanVicente MirabetJosé Manuel García-verdugoCarmen EscobedoCarmen Escobedo-luceaMelissa LezametaCarmen BellverGiancarlo Fortesubject
Cèl·lules mare neuralsCellular differentiationCell- and Tissue-Based TherapyAdipose tissueCell SeparationStem cell markerRegenerative MedicineRegenerative medicine0302 clinical medicineTissue engineeringMolecular Cell BiologyAdipocytesNeurociènciesGene Regulatory Networks0303 health sciencesMultidisciplinaryStem CellsQRGene Expression Regulation DevelopmentalCell Differentiation3. Good healthCell biologyAdult Stem Cells317 Pharmacy030220 oncology & carcinogenesisMedicineStem cellCellular TypesMetabolic Networks and PathwaysResearch ArticleBiotechnologyAdultAdolescentClinical Research DesignScienceCell PotencyPrimary Cell CultureBiologyCell Growth03 medical and health sciencesYoung AdultAnimalsHumansBiology030304 developmental biologyCell ProliferationTissue EngineeringGene Expression ProfilingMesenchymal stem cellCell cultureImmunologyBiomarkersDevelopmental Biologydescription
Human adipose stem cells (hASCs) play a crucial role in the fields of regenerative medicine and tissue engineering for different reasons: the abundance of adipose tissue, their easy harvesting, the ability to multipotent differentiation and the fact that they do not trigger allogeneic blood response or secrete cytokines that act as immunosuppressants. The vast majority of protocols use animal origin reagents, with the underlying risk of transmitting infections by non-human pathogens. We have designed a protocol to isolate and maintain the properties of hASCs avoiding xenogeneic reagents. These changes not only preserve hASCs morphology, but also increase cell proliferation and maintain their stem cell marker profile. On the other hand, human serum albumin (HSA), Tryple® and human Serum (HS), do not affect hASCs multipotent differentiation ability. The amendments introduced do not trigger modifications in the transcriptional profile of hASCs, alterations in key biochemical pathways or malignization. Thus, we have proven that it is possible to isolate and maintain hASCs avoiding animal reagents and, at the same time, preserving crucial culture parameters during long term culture. Thereby we have revealed a novel and effective tool for the improvement of clinical, cell-based therapies. Peer reviewed
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2013-07-09 | PLoS ONE |