6533b861fe1ef96bd12c45b7
RESEARCH PRODUCT
Evidence of cytotoxic activity against mammalian red blood cell of Na+ channel neurotoxin (Ae1) from sea anemone (Actinia equina)
M. ParisiM. TrapaniL. CardinaleM. Cammaratasubject
0301 basic medicine010405 organic chemistryHemolysinActinia equina; Ae1 neurotoxin; Hemolysin; Na+ channel neurotoxin; Sea anemone; Animal Science and ZoologyAe1 neurotoxinSea anemone01 natural sciences0104 chemical sciencesActinia equina03 medical and health sciences030104 developmental biologylcsh:Biology (General)Na channel neurotoxinNa+ channel neurotoxinAnimal Science and Zoologylcsh:QH301-705.5description
The diversification of anthozoan toxins played an important role in the ability to colonize various ecological niches. In this study we evaluated the hemolytic activity of HPLC separated fraction of tentacle extracts of sea anemone Actinia equina. Toxic components from acid tissue tentacle extracts were investigated by size exclusion and reverse phase HPLC to characterize cytolytic molecules. A novel low molecular weight active fraction was sequenced by MALDI TOF analysis and a protein correspondent to 5.4 kDa Sodium channel neurotoxin (Ae1) from A. equina was identified. Synthetic Ae1 was assayed and it showed an hemolytic activity against mammalian erythrocytes in a dose dependent manner. Cytolytic activity in addition to neuro-inhibitory function could be a further property of Ae1 toxin repertoire.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2016-01-01 |