6533b861fe1ef96bd12c468f

RESEARCH PRODUCT

Comprehensive Analysis of Gene Expression Profiles of the Beet Armyworm Spodoptera exigua Larvae Challenged with Bacillus thuringiensis Vip3Aa Toxin

Salvador HerreroBaltasar EscricheYolanda BelJuliana CostaAgata K. Jakubowska

subject

ScienceBacillus thuringiensisSpodopteraSpodopteraBacterial ProteinsBeet armywormBacillus thuringiensisExiguaGene expressionAnimalsMode of actionPest Control BiologicalGeneOligonucleotide Array Sequence AnalysisGeneticsRegulation of gene expressionMultidisciplinarybiologyQfungiRbiology.organism_classificationMolecular biologyLarvaMedicineTranscriptomeResearch Article

description

Made available in DSpace on 2014-12-03T13:11:48Z (GMT). No. of bitstreams: 0 Previous issue date: 2013-12-02Bitstream added on 2014-12-03T13:22:24Z : No. of bitstreams: 1 WOS000327944500105.pdf: 2100332 bytes, checksum: 71ea7616e9a7949eed19cff4ce56111f (MD5) Spanish Ministerio de Ciencia e Innovacion European FEDER funds Generalitat Valenciana Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Host-pathogen interactions result in complex relationship, many aspects of which are not completely understood. Vip proteins, which are Bacillus thuringensis (Bt) insecticidal toxins produced during the vegetative stage, are selectively effective against specific insect pests. This new group of Bt proteins represents an interesting alternative to the classical Bt Cry toxins because current data suggests that they do not share the same mode of action. We have designed and developed a genome-wide microarray for the beet armyworm Spodoptera exigua, a serious lepidopteran pest of many agricultural crops, and used it to better understand how lepidopteran larvae respond to the treatment with the insecticidal protein Vip3Aa. With this approach, the goal of our study was to evaluate the changes in gene expression levels caused by treatment with sublethal doses of Vip3Aa (causing 99% growth inhibition) at 8 and 24 h after feeding. Results indicated that the toxin provoked a wide transcriptional response, with 19% of the microarray unigenes responding significantly to treatment. The number of up-and down-regulated unigenes was very similar. The number of genes whose expression was regulated at 8 h was similar to the number of genes whose expression was regulated after 24 h of treatment. The up-regulated sequences were enriched for genes involved in innate immune response and in pathogen response such as antimicrobial peptides (AMPs) and repat genes. The down-regulated sequences were mainly unigenes with homology to genes involved in metabolism. Genes related to the mode of action of Bt Cry proteins were found, in general, to be slightly overexpressed. The present study is the first genome-wide analysis of the response of lepidopteran insects to Vip3Aa intoxication. An insight into the molecular mechanisms and components related to Vip intoxication will allow designing of more effective management strategies for pest control. Univ Valencia, Dept Genet, Valencia, Spain UNESP, Dept Appl Biol, Jaboticabal, SP, Brazil UNESP, Dept Appl Biol, Jaboticabal, SP, Brazil Spanish Ministerio de Ciencia e InnovacionPBH2008-0113-PC Spanish Ministerio de Ciencia e InnovacionAGL2009-13340-C02-01 Spanish Ministerio de Ciencia e InnovacionAGL2012-39946-C02-01 Generalitat ValencianaGVPrometeo2011-044 CAPES: 194/09

10.1371/journal.pone.0081927http://europepmc.org/articles/PMC3846680