Cilvēka ādas cilmes šūnu neirodiferenciācija in vitro

6533b861fe1ef96bd12c485d

RESEARCH PRODUCT

Cilvēka ādas cilmes šūnu neirodiferenciācija in vitro

Atis Krūmiņš

subject

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Ādas cilmes šūnu neirodiferenciācija un izmantošana terapeitiskos nolūkos, ir ievērojams diskusiju un izpētes objekts. Šī darba mērķis bija gūt labāku ieskatu šūnu neirodiferenciācijas procesos, iegūt iemaņas darbā ar audu kultūru laboratorijā un apskatīt pieejamo literatūru par cilmes šūnām un par iespējām tās izmantot terapeitiskos nolūkos. Darbā izmantojām sekojošās metodes: dermā esošo cilmes šūnu izdalīšana, šūnu pavairošana un uzglabāšanas metodes, neirodiferenciācijas inducēšanas, imunocitoķīmiskā krāsošana (ICĶ), plūsmas citometrija. ICĶ iegūtie dati liecina, ka no dermas iegūtās šūnas ekspresē nestīnu, diferencējot šūnas FEB un samazinātā seruma vidē tās ekspresē nestīnu, tubulīnu B III un GFAP virsmas marķierus. Plūsmas citometrijas dati liecina, ka diferenciācijas laikā šūnās pastiprinās Mitotracker CMX ROS fluorescences intensitāte, kas varētu liecināt, par paaugstinātu mitohondriju membrānas potenciālu.

year	journal	country	edition	language
2011-01-01

https://dspace.lu.lv/dspace/handle/7/20407

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