6533b861fe1ef96bd12c4ef1

RESEARCH PRODUCT

Interaction of polyribosomal components and polyribonucleotides with microtubule proteins

Rudolf K. ZahnH. C. SchröderW. E. G. MüllerAugust Bernd

subject

chemistry.chemical_classificationMessenger RNAPolyribonucleotidesBrainProteinsNerve Tissue ProteinsGeneral MedicinePolymerMatrix (biology)BiologyRibosomeChain lengthchemistryBiochemistryMicrotubulePolyribosomesGeneticsAnimalsCattleNucleotideRNA MessengerPoly AMicrotubule-Associated ProteinsMolecular BiologyPolyribonucleotides

description

To demonstrate the affinity of RNA-containing polyribosomal components (isolated from L5178y cells) to microtubules, microtubule protein was attached to an insoluble matrix. In contrast to ribosomes, poly(A) (+) mRNA and poly(A)-RNP were found to bind to the matrix. Using synthetic polyribonucleotides, no significant differences in the binding properties of single- and double stranded polymers of different base composition to microtubule protein were observed. However, binding is dependent on the size of the polymer; a minimal chain length of 12 nucleotide units is required.

yearjournalcountryeditionlanguage
1982-11-30Molecular Biology Reports
https://doi.org/10.1007/bf00776585