6533b861fe1ef96bd12c561e
RESEARCH PRODUCT
Solution properties of polyelectrolytes. II. Comparison of the effects of both polyion and eluent salt concentrations on the calibrations in aqueous exclusion chromatography
Rosa GarcíaVicente SoriaLorenzo BracoConcepción AbadAgustín Campossubject
chemistry.chemical_classificationGel permeation chromatographyAqueous solutionChromatographyColumn chromatographyChemistryElutionIonic strengthSize-exclusion chromatographyGeneral EngineeringGeneral Materials SciencePolymerPolyelectrolytedescription
The elution behaviour of charged water-soluble polymers (sodium polystyrene sulphonate) in both salt-free and NaCl-containing aqueous mobile phases has been investigated by size-exclusion chromatography. A comparative analysis has been established between the results as a function of polyion and salt concentrations, which demonstrates that the chromatographic behaviour is similar in both cases. The variation of the slopes of the different concentration-dependent calibration curves is discussed in terms of polyelectrolyte conformational changes. Finally, a semi-empirical model has been developed to predict the dependence of the elution volume in either polyion or salt concentration, and its validity has been checked in a range from very dilute to moderate concentrations.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1987-11-01 | British Polymer Journal |