6533b861fe1ef96bd12c5699
RESEARCH PRODUCT
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subject
0301 basic medicineRegulation of gene expressionHippo signaling pathwayanimal structuresMultidisciplinaryGeneral Physics and AstronomyGeneral ChemistryBiologyGeneral Biochemistry Genetics and Molecular BiologyHedgehog signaling pathwayNeural stem cellnervous system diseasesCell biology03 medical and health sciences030104 developmental biologynervous systembiological phenomena cell phenomena and immunitySignal transductionStem cellMitosisreproductive and urinary physiologyDrosophila Proteindescription
AbstractStem cells control their mitotic activity to decide whether to proliferate or to stay in quiescence. Drosophila neural stem cells (NSCs) are quiescent at early larval stages, when they are reactivated in response to metabolic changes. Here we report that cell-contact inhibition of growth through the canonical Hippo signalling pathway maintains NSC quiescence. Loss of the core kinases hippo or warts leads to premature nuclear localization of the transcriptional co-activator Yorkie and initiation of growth and proliferation in NSCs. Yorkie is necessary and sufficient for NSC reactivation, growth and proliferation. The Hippo pathway activity is modulated via inter-cellular transmembrane proteins Crumbs and Echinoid that are both expressed in a nutrient-dependent way in niche glial cells and NSCs. Loss of crumbs or echinoid in the niche only is sufficient to reactivate NSCs. Finally, we provide evidence that the Hippo pathway activity discriminates quiescent from non-quiescent NSCs in the Drosophila nervous system.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2016-01-29 | Nature Communications |