6533b861fe1ef96bd12c59c5

RESEARCH PRODUCT

Expression of a glucocorticoid receptor (DlGR1) in several tissues of the teleost fish Dicentrarchus labrax

Nicolò ParrinelloMonica CeliAiti VizziniGiuseppina SalernoMirella VazzanaM. L. Di Bella

subject

Fish Proteinsmedicine.medical_specialtyBlotting WesternGene ExpressionSpleenIn situ hybridizationBiologyPeritoneal cavityReceptors GlucocorticoidGlucocorticoid receptorInternal medicinemedicineAnimalsRNA MessengerReceptorIn Situ HybridizationRiboprobeCell BiologyGeneral Medicinebiology.organism_classificationImmunohistochemistryMolecular biologymedicine.anatomical_structureEndocrinologyImmunohistochemistryBassDicentrarchusDevelopmental Biology

description

Since glucocorticoids have a role in maintaining the homeostatic status in fish, in the present paper mRNA expression (in situ hybridization) and tissue immunohistochemical localization of a glucocorticoid receptor (DlGR1) in several Dicentrarchus labrax organs are reported. Riboprobe and specific antibodies were prepared by using the DlGR1 that has been previously cloned and sequenced from peritoneal cavity leukocytes. Both mRNA and receptor were identified in head kidney, spleen, gills, intestine, heart and liver tissues. The functional roles of DlGR1 localization are discussed.

yearjournalcountryeditionlanguage
2008-04-01Tissue and Cell
https://doi.org/10.1016/j.tice.2007.09.008