6533b862fe1ef96bd12c6103

RESEARCH PRODUCT

Diminished Inhibition of Adhesion Molecule Expression in Prostacyclin Receptor Desensitized Human Platelets

Juergen MeyerHarald DariusBinz CAndreas FischKerstin Veit

subject

medicine.medical_specialtyChemistryProstacyclinAdhesionThrombinEndocrinologyInternal medicinecardiovascular systemmedicinelipids (amino acids peptides and proteins)PlateletReceptorProstacyclin receptorIC50circulatory and respiratory physiologyIloprostmedicine.drug

description

Long-term exposure of platelets to prostacyclin or iloprost (100nM, 3hr) results in receptor desensitization measured as decrease in 3H-iloprost binding sites by 47 +/- 14%. Desensitized platelets respond with an increased adhesion to endothelial cells. The mechanism of increased adhesiveness was studied by measuring the expression of the adhesion molecule CD62p (p-selectin; GMP140) on washed human platelets by flowcytometry. In thrombin stimulated platelets CD62p expression was dose-dependently reduced by iloprost. In receptor desensitized platelets IC50 for iloprost inhibition of thrombin-induced CD62p expression increased from 0.48 +/- 0.10 to 2.4 +/- 0.7 nM.

yearjournalcountryeditionlanguage
1995-01-01
https://doi.org/10.1007/978-3-0348-7346-8_12