6533b862fe1ef96bd12c6281
RESEARCH PRODUCT
Different response of TH1 cells for stimulation with anti-CD3 antibodies.
Angelika B. Reske-kunzEdgar SchmittMartin RöllinghoffMichael Lohoffsubject
Antigens Differentiation T-LymphocyteCD4-Positive T-LymphocytesCD3 ComplexCell Survivalmedicine.medical_treatmentImmunologyDose-Response Relationship ImmunologicReceptors Antigen T-CellMice Inbred StrainsBiologyLymphocyte Activationchemistry.chemical_compoundMiceAntigenmedicineImmunology and AllergyAnimalsInterleukin 4Cell growthInterleukinAntibodies MonoclonalT helper cellT-Lymphocytes Helper-InducerMolecular biologyCytokinemedicine.anatomical_structurechemistryCell cultureImmunologyPhorbolInterleukin-2Tetradecanoylphorbol AcetateInterleukin-4Spleendescription
In this report, evidence is provided for a further subdivision of CD4+ T helper cell lines. The earlier definition of the TH1 and TH2 subtypes was confirmed by their differential response to interleukin (IL) 1. An additional subdivision of the TH1 subset was revealed when TH1 cell lines were costimulated with anti-CD3 antibodies and IL2. The IL2-induced proliferation of three of the resulting TH1 lines was blocked by anti-CD3 antibodies. By contrast, no such block was observed in a fourth TH1 cell line. In all four lines anti-CD3 triggering caused production of IL2. The block of proliferation was reversed neither by antigen-presenting cells nor by phorbol 12-myristate 13-acetate, a protein kinase C activator. In all TH2 cells, however, anti-CD3 triggering led to IL4 production, but had only marginal effects on IL2-induced cell proliferation.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1990-03-01 | European journal of immunology |