6533b862fe1ef96bd12c6e55
RESEARCH PRODUCT
Developmental expression of two Haliotis asinina hemocyanin isoforms
Bernard M. DegnanKlaus StreitDaniel J. JacksonBernhard Liebsubject
Gene isoformCancer ResearchDNA ComplementaryEmbryo Nonmammalianmedicine.medical_treatmentMolecular Sequence DataVeligermedicineAnimalsProtein IsoformsAmino Acid SequenceImmunoelectrophoresisMolecular BiologyGeneConserved SequenceIn Situ HybridizationPhylogenySequence Homology Amino AcidbiologyHaliotis asininaReverse Transcriptase Polymerase Chain ReactionOxygen transportGene Expression Regulation DevelopmentalHemocyaninCell BiologyAnatomybiology.organism_classificationCell biologyMolluscaLarvaTrochophoreHemocyaninsElectrophoresis Polyacrylamide GelDevelopmental biologyDevelopmental Biologydescription
Hemocyanins are large copper-containing respiratory proteins that play a role in oxygen transport in many molluscs. In some species only one hemocyanin isoform is present while in others two are expressed. The physiological relevance of these isoforms is unclear and the developmental and tissue-specific expression of hemocyanin genes is largely unknown. Here we show that two hemocyanin genes in the gastropod Haliotis asinina, which encode H. asinina hemocyanin (HaH1) and HaH2 isoforms, are developmentally expressed. These genes initially are expressed in a small number of mesenchyme cells at trochophore and pre-torsional veliger stages, with HaH1 expression slightly preceding HaH2. These cells largely are localized to the visceral mass, although a small number of cells are present in head and foot regions. Following metamorphosis the isoforms show overlapping as well as isoform-specific expression profiles, suggesting some degree of isoform-specific function.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2005-09-01 | Differentiation |