6533b862fe1ef96bd12c6e91
RESEARCH PRODUCT
l-[3H]lysine binding to rat retinal membrane: II. effect of kainic acid,d,l-?-aminoadipic acid, iodoacetic acid, and modification by dark-exposure
R. GuarneriFederico PiccoliP. GuarneriF. Pontesubject
MaleKainic acidgenetic structuresIodoacetic acidmedicine.medical_treatmentIntraperitoneal injectionLysineIodoacetates2-Aminoadipic AcidBiologycomplex mixturesBiochemistryRetinaCellular and Molecular Neurosciencechemistry.chemical_compoundmedicineAnimalsVisual PathwaysBinding siteKainic AcidLysineBrainRetinalGeneral MedicineDarknessAmino Acids DicarboxylicIodoacetic AcidRatsKineticschemistryBiochemistryDarknessBiophysicsbacteriasense organs2-Aminoadipic Aciddescription
The rat retina and the different brain regions contain membranes sites that bindl-lysine in the nanomolar range. These binding sites undergo changes in different experimental conditions, thus: I) intraocular injection of kainic acid induces a reduction of the density ofl-lysine binding sites, II)d,l-α-aminoadipic acid injected into the eye enhances both kinetic parameters (Bmax andKd) ofl-[3H]lysine binding sites, III) the intraperitoneal injection of iodoacetic acid decreases the sensitivity for its ligand binding sites, and IV) the exposure to darkness of the rats reducesl-[3H]lysine binding in the retina, thalamus, hypothalamus and superior colliculus, but not in the occipital cortex; such a decrease appears to be characterized, at least in the retina, by a lower sensitivity of the binding sites forl-lysine after the exposure to darkness. The results show thatl-lysine binding sites are located on kainic acid-sensitive cells and can be involved in the physiological mechanism of vision.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1986-12-01 | Neurochemical Research |