Phosphatidylcholine-Gramicidin a Interaction Study in Non-Aqueous Solvent with a new HPLC-SEC Column

6533b862fe1ef96bd12c7489

RESEARCH PRODUCT

Phosphatidylcholine-Gramicidin a Interaction Study in Non-Aqueous Solvent with a new HPLC-SEC Column

Concepción Abad Lorenzo Braco

subject

Solvent chemistry.chemical_compound Aqueous solution Chromatography Column chromatography Resolution (mass spectrometry)chemistry Phosphatidylcholine Molecular Medicine lipids (amino acids peptides and proteins)Biological membrane High-performance liquid chromatography Tetrahydrofuran

description

Abstract A new HPLC-SEC column, Ultrastyragel 500 A, has been assayed in order to check the separation between gramicidin A and phosphatidylcholine in tetrahydrofuran. The good resolution of both compounds has allowed the quantitation of lipid-polypeptide interaction in terms of the binding ratio parameter, BR. A BR value of 3.6 moles of phosphatidylcholine per mole of gramicidin A has been obtained for a 0.1 % (w/v) phosphatidylcholine solution as eluent. The suitability of this support for studies on lipid-polypeptide interactions in non-aqueous solvents is demonstrated.

year	journal	country	edition	language
1985-03-01	Journal of Liquid Chromatography

https://doi.org/10.1080/01483918508067100

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