6533b863fe1ef96bd12c77c5
RESEARCH PRODUCT
Mapping and Quantification of tRNA 2′-O-Methylation by RiboMethSeq
Adeline GalvaninLilia AyadiYuri MotorinVirginie MarchandMark Helmsubject
chemistry.chemical_classification0303 health sciencesTRNA modificationChemistry2'-O-methylationRNA[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biologyComputational biologyRibosomal RNADNA sequencing03 medical and health sciences0302 clinical medicine030220 oncology & carcinogenesisEpitranscriptomics[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]Transfer RNANucleotideComputingMilieux_MISCELLANEOUS030304 developmental biologydescription
Current development of epitranscriptomics field requires efficient experimental protocols for precise mapping and quantification of various modified nucleotides in RNA. Despite important advances in the field during the last 10 years, this task is still extremely laborious and time-consuming, even when high-throughput analytical approaches are employed. Moreover, only a very limited subset of RNA modifications can be detected and only rarely be quantified by these powerful techniques. In the past, we developed and successfully applied alkaline fragmentation-based RiboMethSeq approach for mapping and precise quantification of multiple 2'-O-methylation residues in ribosomal RNA. Here we describe a RiboMethSeq protocol adapted for the analysis of bacterial and eukaryotic tRNA species, which also contain 2'-O-methylations at functionally important RNA regions.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2018-12-12 |