6533b863fe1ef96bd12c7800
RESEARCH PRODUCT
Self-assembling of poly(aspartic acid) with bovine serum albumin in aqueous solutions
Maria BerceaAurica P. ChiriacMihai AsandulesaBernhard A. WolfLoredana E. Nitasubject
Models MolecularProtein ConformationGlobular protein02 engineering and technology010402 general chemistry01 natural sciencesBiochemistryDynamic light scatteringStructural BiologyAspartic acidZeta potentialAnimalsBovine serum albuminMolecular Biologychemistry.chemical_classificationAqueous solutionChromatographybiologyChemistryWaterSerum Albumin BovineGeneral MedicineHydrogen-Ion Concentration021001 nanoscience & nanotechnology0104 chemical sciencesSolutionsDrug deliverybiology.proteinCattlePeptides0210 nano-technologyProtein BindingNuclear chemistryMacromoleculedescription
Abstract Macromolecular co-assemblies built up in aqueous solutions, by using a linear polypeptide, poly(aspartic acid) (PAS), and a globular protein, bovine serum albumin (BSA), have been studied. The main interest was to identify the optimum conditions for an interpenetrated complex formation in order to design materials suitable for biomedical applications, such as drug delivery systems. BSA surface possesses several amino- and carboxylic groups available for covalent modification, and/or bioactive substances attachment. In the present study, mixtures between PAS and BSA were investigated at 37 °C in dilute aqueous solution by viscometry, dynamic light scattering and zeta potential determination, as well as in solid state by AFM microscopy and dielectric spectroscopy. The experimental data have shown that the interpolymer complex formation occurs for a PAS/BSA molar ratio around 0.541.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2016-10-17 | International Journal of Biological Macromolecules |