6533b86cfe1ef96bd12c7fc4

RESEARCH PRODUCT

Investigating the Role of the Microsomal Epoxide Hydrolase Membrane Topology and Its Implication for Drug Metabolism Pathways

Bettina LöllmannFranz OeschThomas FriedbergRomy HollerMichael ArandRoger Becker

subject

Signal peptideHydrolysisBiochemistrybiologyChemistryEndoplasmic reticulumMembrane topologyMicrosomal epoxide hydrolasebiology.proteinCytochrome P450Epoxide hydrolaseDrug metabolism

description

The microsomal epoxide hydrolase (mEH) catalyzes the hydrolysis of reactive epoxides which are formed by the action of cytochromes P450 from xenobiotics. In addition the mEH has been found to mediate the transport of bile acids. For the mEH it has been shown that it is cotranslationally inserted into the endoplasmic reticulum. Here we demonstrate that the amino-terminal twenty amino acid residues of this protein serve as its single membrane anchor signal sequence and that the function of this sequence can be also supplied by a cytochrome P450 (CYP2B1) anchor signal sequence.

yearjournalcountryeditionlanguage
1996-01-01
https://doi.org/10.1007/978-1-4757-9480-9_3