6533b86cfe1ef96bd12c832b

RESEARCH PRODUCT

Influence of oxygen tension on the anti-inflammatory and chondroprotective effects of heme oxygenase-1 in healthy and osteoarthritic human chondrocytes

subject

description

s / Osteoarthritis and Cartilage 20 (2012) S54–S296 S136 their isolation. The absence of cross-reaction with the IIA isoform was established by ELISA andWB. In addition, the Saos-2 cell linewas chosen to test a possible labelling of other fibrillar procollagens, mainly type I, V and XI. In fact, this cell line is described to synthesize the (a1)I, (a2)I, (a1)V, (a2)V, (a1)XI and (a2)XI, but no (a3)XI chains. No signal was detected on WB of cellular extracts or conditioned media with anti-pNIIB52, whereas antibodies to the collagen I, V and XI triple-helical parts revealed indeed the presence of proforms of these collagens. Conclusions: Anti-pNIIB52 antibodies allow a very sensitive and specific detection of the procollagen IIB, the protein synthesized by mature chondrocytes. No cross-reactions with the IIA or with the major fibrillar procollagens synthesized by cells with an osteoblastic phenotype were observed. These antibodies offer a new tool in basic aswell as in translational research concerning cartilage differentiation, homeostasis and repair. 262 INFLUENCE OF OXYGEN TENSION ON THE ANTI-INFLAMMATORY AND CHONDROPROTECTIVE EFFECTS OF HEME OXYGENASE-1 IN HEALTHY AND OSTEOARTHRITIC HUMAN CHONDROCYTES M. Guillen , V. Clerigues , F. Gomar , C.L. Murphy , M. Alcaraz . Univ. of Valencia, Burjasot, Valencia, Spain; CEU Cardenal Herrera Univ., Moncada, Spain; Univ. of Valencia, Burjasot, Valencia, Spain; Univ. of Valencia, Valencia, SPAIN; Kennedy Inst. of Rheumatology, London, United