6533b86cfe1ef96bd12c8a2c
RESEARCH PRODUCT
Cell wall xyloglucan incorporation by xyloglucan endotransglucosylase/hydrolase in pine hypocotyls
Ester P. Lorencessubject
chemistry.chemical_classificationbiologyfungifood and beveragesPlant ScienceGeneral MedicineEnzyme assayHypocotylCell wallXyloglucanchemistry.chemical_compoundchemistryBiochemistryAuxinGlycosyltransferaseGeneticsbiology.proteinXyloglucan:xyloglucosyl transferaseAgronomy and Crop ScienceGlucandescription
Abstract In the present study, we have examined the incorporation of labelled xyloglucan oligosaccharides into the cell wall xyloglucan of pine hypocotyl sections, and the effect of plant hormones on the incorporation and on XTH enzyme activity (EC 2.4.1.207). Xyloglucan heptasaccharide [ 3 H ]XXXGol was absorbed, transported and incorporated into the cell wall xyloglucan of de-rooted pine hypocotyls. Incorporation of [ 3 H ]XXXGol into soluble and cell wall xyloglucan was induced by IAA, brassinolides and XXXG in pine hypocotyl segments. A relationship between growth induction, by IAA, brassinolides and XXXG, and both soluble and cell wall-bound XTH was found. We suggest that IAA induced-growth might be related with an increase in the activity of an XTH with a preference for using xyloglucan oligosaccharides, instead polymeric xyloglucan, as acceptors of the endotransglucosylation reactions.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2004-05-01 | Plant Science |