6533b86cfe1ef96bd12c8a7f
RESEARCH PRODUCT
Mannose-Decorated Multicomponent Supramolecular Polymers Trigger Effective Uptake into Antigen-Presenting Cells
Hajime YurugiEdgar SchmittDaniel SpitzerNatascha StergiouDavid StraßburgerDieter SchollmeyerPol BeseniusMoritz Urschbachsubject
Models MolecularDendrimersMannosidesBiocompatibilitySupramolecular chemistryAntigen-Presenting Cells010402 general chemistry01 natural sciencesBiochemistryPolyethylene GlycolsMiceSurface-Active Agentschemistry.chemical_compoundAmphiphileAnimalsAntigen-presenting cellMolecular BiologyCells CulturedFluorescent Dyeschemistry.chemical_classificationMicroscopy Confocal010405 organic chemistryMacrophagesOrganic ChemistryBiological TransportCarbocyaninesCell sorting0104 chemical sciencesSupramolecular polymerschemistryMannosidesBiophysicsMolecular MedicinePeptidesEthylene glycoldescription
A modular route to prepare functional self-assembling dendritic peptide amphiphiles decorated with mannosides, to effectively target antigen-presenting cells, such as macrophages, is reported. The monomeric building blocks were equipped with tetra(ethylene glycol)s (TEGs) or labeled with a Cy3 fluorescent probe. Experiments on the uptake of the multifunctional supramolecular particles into murine macrophages (Mφs) were monitored by confocal microscopy and fluorescence-activated cell sorting. Mannose-decorated supramolecular polymers trigger a significantly higher cellular uptake and distribution, relative to TEG carrying bare polymers. No cytotoxicity or negative impact on cytokine production of the treated Mφs was observed, which emphasized their biocompatibility. The modular nature of the multicomponent supramolecular polymer coassembly protocol is a promising platform to develop fully synthetic multifunctional vaccines, for example, in cancer immunotherapy.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2018-02-27 | ChemBioChem |