6533b86cfe1ef96bd12c8ab8
RESEARCH PRODUCT
Ci8 short, a novel LPS-induced peptide from the ascidian Ciona intestinalis,modulates responses of the human immune system
Aiti VizziniAlessandra LongoFrank A. SchildbergMario MelisFrancesco GervasiAngela BonuraPaolo ColomboSara Vlahsubject
CD4-Positive T-LymphocytesLipopolysaccharides0301 basic medicineUntranslated regionImmunologyReceptors Antigen T-CellLymphocyte ActivationMajor histocompatibility complexInterferon-gamma03 medical and health sciences0302 clinical medicineImmune systemAnimalsHumansImmunology and AllergyCiona intestinalisClonal Selection Antigen-Mediated3' Untranslated RegionsCells CulturedCell ProliferationGeneticsZAP-70 Protein-Tyrosine KinaseInnate immune systembiologyThree prime untranslated regionT-cell receptorHematologyAcquired immune systembiology.organism_classificationHuman PBMCs Adaptive immunityT cellsImmunity InnateCiona intestinalisCell biology030104 developmental biologyLeukocytes Mononuclearbiology.proteinAntimicrobial Cationic Peptides030215 immunologydescription
The selective modulation of immunity is an emerging concept driven by the vast advances in our understanding of this crucial host defense system. Invertebrates have raised researchers’ interest as potential sources of new bioactive molecules owing to their antibacterial, anticancer and immunomodulatory activities. A LipoPolySaccharide (LPS) challenge in the ascidian Ciona intestinalis generates the transcript, Ci8 short, with cisregulatory elements in the 3′ UTR region that are essential for shaping innate immune responses. The derived amino acidic sequence in silico analysis showed specific binding to human Major Histocompatibility Complex (MHC) Class I and Class II alleles. The role of Ci8 short peptide was investigated in a more evolved immune system using human Peripheral Blood Mononuclear Cells (PBMCs) as in vitro model. The biological activities of this molecule include the activation of 70 kDa TCR ζ chain Associated Protein kinase (ZAP-70) and T Cell Receptor (TCR) Vβ oligo clonal selection on CD4+ T lymphocytes as well as increased proliferation and IFN-γ secretion. Furthermore Ci8 short affects CD4+/CD25high induced regulatory T cells (iTreg) subset selection which co-expressed the functional markers TGF-β1/Latency Associated Protein (LAP) and CD39/CD73. This paper describes a new molecule that modulates important responses of the human adaptive immune system.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-05-10 |