Simple high-performance liquid chromatographic assay for polyamines and their monoacetyl derivatives.

6533b86cfe1ef96bd12c8b46

RESEARCH PRODUCT

Simple high-performance liquid chromatographic assay for polyamines and their monoacetyl derivatives.

Maria Rita Schiavo Gennaro Taibi

subject

Chromatography Calibration curve Organic solvent Extraction (chemistry)Reproducibility of Results Acetylation General Chemistry Chick Embryo Diamines High-performance liquid chromatography Retina chemistry.chemical_compound Benzoyl chloride chemistry Polyamines Animals Spectrophotometry Ultraviolet Derivatization Quantitative analysis (chemistry)Chromatography High Pressure Liquid

description

A rapid reversed-phase high-performance liquid chromatographic method, using pre-column derivatization with benzoyl chloride and ultraviolet detection at 254 nm, was developed for the simultaneous measurement of polyamines and their monoacetyl derivatives. Calibration curves were linear for concentrations from 1.25 to 25 nmol/ml. The method was employed to assay these compounds in chick embryo retina explants using organic solvent extraction and 1,7-diaminoheptane as an internal standard. This simple and sensitive method can be applied to routine determinations of these compounds in various biological samples.

year	journal	country	edition	language
1993-04-01	Journal of chromatography

10.1016/0378-4347(93)80235-v https://pubmed.ncbi.nlm.nih.gov/8496276

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