6533b86cfe1ef96bd12c8c9f

RESEARCH PRODUCT

In Situ Characterization of Hfq Bacterial Amyloid: A Fourier-Transform Infrared Spectroscopy Study

Valeria MilitelloVéronique ArluisonDavid PartoucheAndrea Gómez-zavagliaFrank WienChristophe Sandt

subject

Microbiology (medical)AmyloidOtras Ingenierías y Tecnologíasprotein fibrillation inhibitionVirulencelcsh:MedicineINGENIERÍAS Y TECNOLOGÍASmedicine.disease_causeArticleHFQHfq03 medical and health sciencesIn vivoGene expressionmedicineImmunology and Allergybacterial amyloidSerum amyloid Aprotein fibrilsMolecular BiologyEscherichia coli030304 developmental biology//purl.org/becyt/ford/2.11 [https]Hfq protein0303 health sciencesGeneral Immunology and Microbiologybiology030306 microbiologyChemistrylcsh:Rfunctional amyloidbiology.organism_classificationCell biologyInfectious Diseases//purl.org/becyt/ford/2 [https]FTIRbiology.proteinBacteria

description

Hfq is a bacterial protein that regulates gene expression at the post-transcriptional level in Gram-negative bacteria. We have previously shown that Escherichia coli Hfq protein, and more precisely its C-terminal region (CTR), self-assembles into an amyloid-like structure in vitro. In the present work, we present evidence that Hfq unambiguously forms amyloid structures also in vivo. Taking into account the role of this protein in bacterial adaptation and virulence, our work opens possibilities to target Hfq amyloid self-assembly and cell location, with potential to block bacterial adaptation and treat infections. Fil: Partouche, David. Centre National de la Recherche Scientifique; Francia. Synchrotron SOLEIL; Francia. Université Paris-Saclay; Francia Fil: Militello, Valeria. Università degli Studi di Palermo; Italia Fil: Gomez Zavaglia, Andrea. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina Fil: Wien, Frank. Synchrotron SOLEIL; Francia Fil: Sandt, Christophe. Synchrotron SOLEIL; Francia Fil: Arluison, Veronique. Centre National de la Recherche Scientifique; Francia. Université Paris Diderot - Paris 7; Francia. Université Paris-Saclay; Francia

yearjournalcountryeditionlanguage
2019-03-01Pathogens
10.3390/pathogens8010036http://www.mdpi.com/2076-0817/8/1/36