6533b86dfe1ef96bd12c956c
RESEARCH PRODUCT
A selective medium for the isolation of malolactic mutants of Leuconostoc oenos
Isabel PardoSergi FerrerManuel Zúñigasubject
MutationbiologyMutantfood and beveragesbiology.organism_classificationmedicine.disease_causeIsolation (microbiology)StreptococcaceaeApplied Microbiology and BiotechnologyMalolactic enzymeMicrobiologyBiochemistrymedicineMalolactic fermentationLeuconostocBacteriadescription
We have developed a selective medium for the isolation of Leuconostoc oenos mutants defective in malolactic fermentation. Forty per cent of colonies isolated directly on selective plates after UV mutagenesis had lost their ability to degrade malate. None of the tested mutants showed any detectable malolactic activity and all lacked a protein band corresponding in size to that of the malolactic enzyme. The availability of such mutants provides a valuable tool both for physiological and genetic research on malolactic fermentation.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1994-12-01 |