6533b86dfe1ef96bd12c9d67
RESEARCH PRODUCT
Chiral separation of ephedrines by liquid chromatography using β-cyclodextrins
Pilar Campí Ns-falcóRosa Herráez-hernándezsubject
chemistry.chemical_classificationChromatographyCyclodextrinBiochemistryAnalytical ChemistryNasal decongestantChiral column chromatographySolventchemistryIonic strengthmedicineEnvironmental ChemistryEphedrineEnantiomerChiral derivatizing agentSpectroscopymedicine.drugdescription
β-cyclodextrins (β-CDs) have been evaluated as chiral recognition agents for the enantioseparation of ephedrines by liquid chromatography (LC). The compounds tested were norephedrine, ephedrine, pseudoephedrine, N-methylephedrine and N-methylpseuoephedrine. Two possibilities have been investigated, the employment of a chiral stationary phase consisting of immobilized β-cyclodextrin, and the addition of the chiral selector to the mobile phase. In the latter approach the following chiral agents have been assayed, native β-cyclodextrin (β-CD), methyl-β-cyclodextrin (M-β-CD), carboxyethyl-β-cyclodextrin (CE-β-CD), carboxymethyl-β-cyclodextrin (CM-β-CD) and hydroxypropyl-β-cyclodextrin (HP-β-CD). The experimental factors affecting the chiral separations have been examined, including the percentage of organic solvent, the ionic strength and pH of the buffer, the type and concentration of chiral selector, and the mobile-phase flow rate. Differences in enantioselectivity displayed by the different methods are discussed.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2001-05-01 | Analytica Chimica Acta |