6533b86dfe1ef96bd12c9dde
RESEARCH PRODUCT
The Separation of High and Low Molecular Weight RNA by Precipitation with N-Cetyl- N,N,N-trimethyl-ammoniumbromide
Barbara SchöbelRudolf K. ZahnGerhard Seibertsubject
ChromatographyPrecipitation (chemistry)ChemistryRNAGeneral Biochemistry Genetics and Molecular Biologydescription
Abstract RNA with a sedimentation constant of 28S and 18S can easily be separated from 4S and 5S RNA. The method depends on the different solubulities of nucleic acids in solutions of the cationic detergent N-cetyl-N,N,N-trimethyl-ammoniumbromide at various ionic strengths. The separation can be achieved with high efficiency in one step.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1977-04-01 | Zeitschrift für Naturforschung C |