6533b86dfe1ef96bd12c9f59
RESEARCH PRODUCT
Modulation of Base Excision Repair Alters Cellular Sensitivity to UVA1 but not to UVB¶
Bernd KainaSabine GröschIndraneel ChakrabartyKatherine J. KimThomas M. RüngerGuang-zhi Lisubject
DNA damageChinese hamster ovary cellCellPyrimidine dimerGeneral MedicineBase excision repairBiologyBiochemistryMolecular biologychemistry.chemical_compoundmedicine.anatomical_structurechemistryPhotosensitivityBiochemistryDownregulation and upregulationMethoxyaminemedicinePhysical and Theoretical Chemistrydescription
Abstract Oxidative DNA damage has been implicated in some of the biological properties of UVA but so far not in the acute photosensitivity or cellular sensitivity. In contrast to pyrimidine dimers, oxidative DNA damage is predominantly processed by base excision repair (BER). In order to further clarify the role of oxidative DNA damage and its repair in the acute cellular response to UV light, we studied UVA1 and UVB sensitivities in three different cell model systems with modified BER. 8-Oxoguanine-DNA-glycosylase 1–/– (OGG1–/–) mouse embryonal fibroblasts and human fibroblasts in which BER was inhibited by incubation with methoxyamine were hypersensitive to UVA1, in particular to low doses. This hypersensitivity could be partially corrected by reexpression of OGG1 in OGG1–/– cells. The Chinese hamster ovary (CHO) cells with upregulated AP-endonuclease 1 exhibited reduced UVA1 sensitivity. UVB sensitivity was not altered in any of the cell models. These results indicate that DNA damage, in particular oxi...
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2007-05-01 | Photochemistry and Photobiology |