6533b86dfe1ef96bd12ca0f3
RESEARCH PRODUCT
Isolation of the DNA minisatellite probe MZ 1.3 and its application to DNA ‘fingerprinting’ analysis
E. BohnkeU. SchackerCh. RittnerB. HoltkampRolf FimmersH.-h SonnebornPeter M. Schneidersubject
Molecular Sequence DataRestriction MappingDNA SatelliteHomology (biology)Pathology and Forensic MedicineRestriction fragmentchemistry.chemical_compoundHumansGenomic libraryGeneRepetitive Sequences Nucleic AcidGeneticsGenomic LibraryBase SequencebiologyNucleotide MappingDNAMolecular biologyBlotting SouthernVariable number tandem repeatMinisatelliteDNA profilingchemistrybiology.proteinDNA ProbesLawPolymorphism Restriction Fragment LengthDNAdescription
Abstract A minisatellite probe, MZ 1.3, detecting hypervariable fragment patterns was isolated from a human genomic library. A repetitive sequence of 27 bp length was identified which is contained in the probe approx. 40 times. The MZ 1.3 repeat shows variable homology of 53–73% to the repetitive sequence of the protein III gene of the bacteriophage M13 genome. Polymorphic restriction fragment patterns were found with MZ 1.3 using the enzymes Hinf I, BstN I, Hae III, Mbo I, PstI PvuII , and Rsa I. An average of 18 polymorphic fragments was observed using Hinf I as enzyme. The band sharing frequency after Hinf I digestion among unrelated individuals was determined to be 23.8 ± 7.2%. An example for the application of MZ 1.3 to paternity testing in an incest case is given. The probe can be used with radioactive or non-radioactive detection systems. An approach is presented to compare polymorphic fragment patterns from individuals obtained by independent gel runs on the basis of relative band positions (RBP) and calculated in a computerized analysis.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1990-02-01 | Forensic Science International |