6533b86dfe1ef96bd12ca89d

RESEARCH PRODUCT

Uptake of Acetylcholine by Bean Hypocotyl Hooks

Elmar Hartmann

subject

biologyDirect effectsfood and beveragesGeneral Medicinebiology.organism_classificationAcetylcholinesteraseHypocotylchemistry.chemical_compoundHydrolysischemistryBiochemistryEtiolationmedicinePhaseolusIncubationAcetylcholinemedicine.drug

description

Summary The uptake of acetylcholine by etiolated hypocotyl hooks of beans ( Phaseolus vulgaris L.) is influenced by light and the pH-value of the incubation medium. Acetylcholine is hydrolysed up to 90 % during the uptake. The hydrolysis is inhibited by pH-value lower than 6.0 and by eserine, an inhibitor of the acetylcholinesterase. The high amount of hydrolysis is a serious problem in experiments involving acetylcholine and necessitates the inclusions of adequate controls without which direct effects of acetylcholine cannot be distinguished from reactions of its metabolic products.

https://doi.org/10.1016/s0044-328x(78)80267-0