6533b86efe1ef96bd12cb4c0

RESEARCH PRODUCT

GAS CHROMATOGRAPHIC ESTIMATION OF ACETYLCHOLINE IN THE RABBIT HEART USING A NITROGEN SELECTIVE DETECTOR

H. Kilbinger

subject

MaleChromatography GasHeart VentriclesCoefficient of variationSodiumchemistry.chemical_elementBlood PressureBiochemistryCholineCellular and Molecular Neurosciencechemistry.chemical_compoundMethodsmedicineAnimalsBioassayCholineHeart AtriaPerchloric acidButyrylcholineChromatographyChemistryMyocardiumAcetylcholineRatsButyratesBiological AssayFemaleRabbitsGas chromatographyPropionatesAcetylcholinemedicine.drug

description

The distribution of ACh in the rabbit heart was investigated by a modified gas chromatographic estimation method. ACh was extracted with perchloric acid, precipitated as reineckate and demethylated with sodium benzenethiolate. The tertiary amines derived from ACh and other choline esters were concentrated by a microdistillation procedure. Gas chromatography was performed using a nitrogen selective detector. In the range of concentrations between 0.4 and 2.5 nmol ACh per tissue sample the coefficient of variation was 5.2 per cent. The recovery of ACh added to heart extracts was 101 per cent. Evidence for the identity of the choline ester isolated from rabbit hearts and authentic ACh was obtained by equal retention times and by correspondence of the ratio N/C of the respective tertiary amines. Parallel measurements using gas chromatography and bioassay on the rat blood pressure yielded closely corresponding values of ACh levels in the rabbit heart. The concentration of ACh was much higher in the atria than in the ventricles. In both atria, and ventricles the ACh concentration was higher in the right than in the left part of the rabbit heart. Endogenous propionylcholine or butyrylcholine were not detected.

yearjournalcountryeditionlanguage
1973-08-01Journal of Neurochemistry
https://doi.org/10.1111/j.1471-4159.1973.tb04261.x