Borrelia spielmanii ārējās virsmas proteīna BSE31 ekspresija un attīrīšana, kristalizācija un struktūras analīze

6533b86efe1ef96bd12cb8ed

RESEARCH PRODUCT

Borrelia spielmanii ārējās virsmas proteīna BSE31 ekspresija un attīrīšana, kristalizācija un struktūras analīze

Elvijs Tarasovs

subject

ārējās virsmas proteīni Laima slimība proteīnu ekspresija Borrelia spielmanii Bioloģija proteīnu attīrīšana

description

Borrelia spielmanii ir ērču pārnēsāta spirohetu dzimtai piederoša baktērija, kas zināma kā Laima slimības ierosinātājs. Ērču barošanās laikā baktērijai nonākot zīdītāja organismā, daudziem tās proteīniem novēro ekspresijas pieaugumu. Viens no tādiem proteīniem ir B.burgdorferi B31 ārējās virsmas proteīns BBE31, kurš ir ļoti svarīgs Laima slimības patoģenēzē, jo bez šī proteīna borēlija nespēj izkļūt no ērces gremošanas sistēmas, lai tālāk nokļūtu jaunajā saimniekorganismā. Darba mērķis ir iegūt B. burgdorferi BBE31 homologo ārējās virsmas proteīnu BSE31 no B. spielmanii ekspresējot to Escherichia coli šūnās, kā arī veikt kristalizāciju un iegūt proteīna kristālstruktūru. Darba gaitā tika sekmīgi iegūts un attīrīts B. spielmanii ārējās virsmas proteīns BSE31. Pēc proteīna kristalizācijas tika savākti difrakcijas dati un iegūta proteīna kristālstruktūra. Bakalaura darbs tika izstrādāts Latvijas Biomedicīnas pētījumu un studiju centrā, Dr. biol. Kalvja Branguļa vadībā.

year	journal	country	edition	language
2018-01-01

https://dspace.lu.lv/dspace/handle/7/38636

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