6533b86efe1ef96bd12cba89

RESEARCH PRODUCT

Adaptive evolution to improve acid tolerance in Oenococcus oeni

subject

description

International audience; Oenococcus oeni is a lactic acid bacterium (LAB) mainly responsible for the malolactic fermentation (MLF) inwine. MLF plays an important role in determining the final quality of wines [1]. Even though this LAB is naturallypresent in musts, wines and oenological environment, spontaneous MLF are usually unpredictable because of thestressful conditions and especially due to acidity [2]. The consequence of the mismanagement of this step might leadto the depreciation of wine quality. To obtain a clone more tolerant to acidity, we undertook a replication of O. oeni until 450 generations in a temporally varying environment (pH 5.3 to 2.9) to improve acid tolerance. To discriminate stress tolerance ofevolved populations versus parental strain an acid stress was performed to both population.