6533b86efe1ef96bd12cbdfe

RESEARCH PRODUCT

Electrochemical identification of toxigenic fungal species using solid-state voltammetry strategies.

José-vicente Gimeno-adelantadoR. Mateo-castroEva M. MateoJosé V. GómezMisericordia JiménezAntonio Doménech-carbóNoemí Montoya

subject

0301 basic medicineFusarium030106 microbiologyAnalytical chemistryFood chemistryGlassy carbonElectrochemistry01 natural sciencesAnalytical Chemistry03 medical and health sciencesScanning electrochemical microscopyFusariumElectrochemistryVitisVoltammetryAspergillusMicroscopyChromatographyAqueous solutionbiology010401 analytical chemistryfood and beveragesGeneral MedicineMycotoxinsbiology.organism_classification0104 chemical sciencesAspergillusFood MicrobiologyEdible GrainFood Science

description

An electrochemical methodology for the characterization of mycotoxin-producing fungal species from the genera Aspergillus and Fusarium using solid-state voltammetry is described. Upon attachment of fungal colony microsamples to glassy carbon electrodes in contact with aqueous acetate buffer, characteristic voltammetric signals mainly associated to the oxidation of polyphenolic metabolites are recorded. The possibility of fungi-localized electrochemical processes was assessed by means of electron microscopy and field emission scanning electrochemical microscopy coupled to the application of oxidative potential inputs. Using pattern recognition methods, the determined voltammetric profiles were able to discriminate between mycotoxin-producing fungi from different sections and to identify selected toxigenic species of the Aspergillus and Fusarium genera isolated from grapes and cereals.

10.1016/j.foodchem.2017.02.033https://pubmed.ncbi.nlm.nih.gov/29934194