6533b86efe1ef96bd12cbe79

RESEARCH PRODUCT

Novel Antihypertensive Lactoferrin-Derived Peptides Produced by Kluyveromyces marxianus: Gastrointestinal Stability Profile and In Vivo Angiotensin I-Converting Enzyme (ACE) Inhibition

Isidra RecioMaría Castelló-ruizPaloma ManzanaresAurora García-tejedorLaura Sánchez-riveraJuan B. Salom

subject

MaleAntihypertensive effectsAdministration OralAngiotensin-Converting Enzyme InhibitorsBlood PressurePeptideLactoferrin-derived peptidesPeptidyl-Dipeptidase AKluyveromycesKluyveromyces marxianusIn vivoRats Inbred SHRRenin–angiotensin systemAnimalsHumansKluyveromyces marxianusAmino Acid SequencePeptide sequenceAntihypertensive AgentsBiotransformationchemistry.chemical_classificationbiologyLactoferrinGastrointestinal digestionGeneral Chemistrybiology.organism_classificationAngiotensin IIRatsLactoferrinEnzymeBiochemistrychemistryHypertensionbiology.proteinCattlePeptidesGeneral Agricultural and Biological SciencesIn vivo ACE inhibition

description

Novel antihypertensive peptides released by Kluyveromyces marxianus from bovine lactoferrin (LF) have been identified. K. marxianus LF permeate was fractionated by semipreparative high performance liquid chromatography and 35 peptides contained in the angiotensin I-converting enzyme (ACE)-inhibitory fractions were identified by using an ion trap mass spectrometer. On the basis of peptide abundance and common structural features, six peptides were chemically synthesized. Four of them (DPYKLRP, PYKLRP, YKLRP, and GILRP) exerted in vitro inhibitory effects on ACE activity and effectively decreased systolic blood pressure after oral administration to spontaneously hypertensive rats (SHRs). Stability against gastrointestinal enzymes suggested that the sequence LRP could contribute to the in vivo effects of parental peptides. Finally, there were reductions in circulating ACE activity and angiotensin II level in SHRs after either DPYKLRP or LRP intake, thus confirming ACE inhibition as the in vivo mechanism for their antihypertensive effect.

yearjournalcountryeditionlanguage
2014-01-31
10.1021/jf4053868http://hdl.handle.net/10261/148419