6533b86efe1ef96bd12cbe8d
RESEARCH PRODUCT
Interleukin-15, as Interferon-gamma, Induces the Killing of Leishmania infantum in Phorbol-Myristate-Acetate-Activated Macrophages Increasing Interleukin-12
Giustina VitaleG. Di BellaSalvatore MilanoViviana FerlazzoPietro D'agostinoSerafino MansuetoN. ChifariEnrico CillariM. La RosaFrancesco ArcoleoR. Carusosubject
medicine.drug_classmedicine.medical_treatmentImmunologyMonoclonal antibodyNeutralizationMicrobiologyInterferon-gammaMicemedicineAnimalsInterferon gammaLeishmania infantumInterleukin-15biologyActivator (genetics)MacrophagesGeneral Medicinebiology.organism_classificationInterleukin-12CytokineInterleukin 15Interleukin 12Leishmaniasis VisceralTetradecanoylphorbol AcetateLeishmania infantummedicine.drugdescription
The potential leishmanicidal activity of interleukin-15 (IL-15) was examined while priming with the cytokine phorbol-myristate-acetate (PMA)-activated macrophages and infecting them with Leishmania infantum parasites. The activation of macrophage cultures with IL-15 determined a significant anti-leishmanial activity, comparable with that induced by interferon-gamma (IFN-gamma). The killing of Leishmania in macrophages primed with IL-15, as well as with IFN-gamma, was followed by an increase in the IL-12 synthesis. The neutralization of IL-15 or IFN-gamma, by specific monoclonal antibodies (MoAb) caused a significant reduction in leishmanicidal activity. Furthermore, in PMA-activated macrophages, the neutralization of IL-12 production by a specific anti-IL-12 MoAb reduced leishmanicidal activity induced by IL-15 and IFN-gamma. Data indicate that IL-15 could have a role as an activator of leishmanicidal activity, directly or indirectly, by inducing IL-12 production.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2004-12-09 | Scandinavian Journal of Immunology |