6533b86efe1ef96bd12cc461

RESEARCH PRODUCT

Caractérisation moléculaire de l’évolution des communautés microbiennes de sols du dispositif EFELE sur la période 2012-2021 : impact de l'apport de PROs et du mode de travail du sol

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It is becoming increasingly difficult to feed the growing population and it is therefore important to doeverything possible to improve our agriculture. To do this, a device called "EFELE" was created in 2012 toobserve how the soil reacts over a period of 20 years. This is essential for cultivation and allows us to obtainvarious information, including bio-indicators. The years 2012 and 2021 will be treated in order to define theamount of DNA in the soil, the microbial balance, the richness and diversity of the soil. In order to study howagriculture could be improved, different trials have been established. One of them studies the effects of theinput of organic waste products (OWPs) from livestock effluents. The other studies the interaction betweentillage method and effluent input. From these amendments, different soils are taken and processed. Theyare prepared for analysis, i.e. sieved and freeze-dried. The same mass of soil is then taken to obtain asupernatant after lysis. The supernatant is then deproteinised to collect the raw DNA, part of which is usedto obtain the amount of DNA in the soil, and the other part is purified to obtain clean DNA to determine themicrobial balance, diversity and richness by Illumina sequencing on 16S and 18S genes. The results of thesamples are not all obtained, however an effect due to the PROs input is observable as well as an effect dueto the simplified work. The most damaging amendment for the soil is mineral nitrogen in the other side cattlemanure seems to be the most efficient.