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RESEARCH PRODUCT
Elicitation of tobacco cells with ergosterol activates a signal pathway including mobilization of internal calcium
Ladislav HavelVladimír MikešTomas KasparovskyClaude HumbertMarie-louise MilatJean-pierre Bleinsubject
0106 biological sciences0303 health sciencesRuthenium redErgosterolbiologyVoltage-dependent calcium channelPhysiologyNicotiana tabacumchemistry.chemical_elementPlant ScienceCalciumbiology.organism_classification01 natural sciencesElicitor03 medical and health scienceschemistry.chemical_compoundCytosolchemistryBiochemistrypolycyclic compoundsGeneticsInositol030304 developmental biology010606 plant biology & botanydescription
Abstract Ergosterol interacts with tobacco suspension ( Nicotiana tabacum ) cells and triggers pH changes of extracellular medium, oxidative burst and synthesis of phytoalexins. Compared with the responses induced by cryptogein, a proteinaceous elicitor from Phytophthora sp., oxidative burst and ΔpH changes were weaker whereas phytoalexin accumulation was higher with ergosterol. Cryptogein stimulated an apparent continuous uptake of external calcium within 40 min, whereas no net uptake of external calcium occurred upon the addition of ergosterol. However, the elicitation with both cryptogein and ergosterol resulted in an increase of the fluorescence of calcium green 1 in cytosol. The use of several inhibitors of calcium channels (La 3+ , TMB-8, verapamil, ruthenium red, nifedipine) and a protein-kinase inhibitor (staurosporin) suggests that the elicitation with ergosterol includes the mobilization of internal calcium stores mediated by inositol 1,4,5-trisphosphate and serine/threonine protein kinases.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2003-05-01 | Plant Physiology and Biochemistry |