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RESEARCH PRODUCT
Exposure assessment approach through mycotoxin/creatinine ratio evaluation in urine by GC–MS/MS
Juan Carlos MoltóJordi MañesYelko Rodríguez-carrascoHouda Berradasubject
AdultMaleTolerable daily intakeChromatography GasAdolescentUrinary systemFood ContaminationPilot ProjectsUrineToxicologymedicine.disease_causeYoung Adultchemistry.chemical_compoundLimit of DetectionTandem Mass SpectrometrymedicineHumansFood scienceChildMycotoxinExposure assessmentCreatinineChromatographyChemistryToxinReproducibility of Resultsfood and beveragesGeneral MedicineMycotoxinsT-2 ToxinCreatinineFood MicrobiologyFemaleGas chromatography–mass spectrometryTrichothecenesFood Sciencedescription
Abstract In this pilot survey human urine samples were analyzed for presence of 15 mycotoxins and some of their metabolites using a novel urinary multi-mycotoxin GC–MS/MS method following salting-out liquid–liquid extraction. Fifty-four urine samples from children and adults residents in Valencia were analyzed for presence of urinary mycotoxin and expressed in gram of creatinine. Three out of 15 mycotoxins were detected namely, HT-2 toxin, nivalenol and deoxynivalenol (DON). 37 samples showed quantifiable values of mycotoxins. Co-occurrence of these contaminants was also observed in 20.4% of assayed samples. DON was the most frequently detected mycotoxin (68.5%) with mean levels of 23.3 μg/g creatinine (range: 2.8–69.1 μg/g creatinine). The levels of urinary DON were used to carry out an exposure assessment approach. 8.1% of total subjects were estimated to exceed the DON provisional maximum tolerable daily intake (PMTDI) (1 μg/kg b.w.). Two out of 9 exposed children exceeded the DON PMTDI thus, making them the most exposed based on the urinary results.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2014-04-14 | Food and Chemical Toxicology |