6533b86efe1ef96bd12cc7dc

RESEARCH PRODUCT

Unraveling the In Vivo Protein Corona

Stephan GehringKatharina LandfesterGabor KuhnGabor KuhnMichael FichterJohanna SimonJohanna SimonVolker MailänderVolker Mailänder

subject

BiodistributionProtein CoronaCell Communication02 engineering and technology010402 general chemistry01 natural sciencesArticleMiceprotein coronaIn vivoAnimalsTissue DistributionMagnetite Nanoparticleslcsh:QH301-705.5biodistributionplasmaWhole bloodChemistrynanoparticleGeneral Medicine021001 nanoscience & nanotechnologyBlood proteinsIn vitro0104 chemical sciencesMice Inbred C57BLin vivoRAW 264.7 Cellslcsh:Biology (General)ProteomeBiophysics0210 nano-technologyserumEx vivo

description

Understanding the behavior of nanoparticles upon contact with a physiological environment is of urgent need in order to improve their properties for a successful therapeutic application. Most commonly, the interaction of nanoparticles with plasma proteins are studied under in vitro conditions. However, this has been shown to not reflect the complex situation after in vivo administration. Therefore, here we focused on the investigation of magnetic nanoparticles with blood proteins under in vivo conditions. Importantly, we observed a radically different proteome in vivo in comparison to the in vitro situation underlining the significance of in vivo protein corona studies. Next to this, we found that the in vivo corona profile does not significantly change over time. To mimic the in vivo situation, we established an approach, which we termed &ldquo

https://doi.org/10.3390/cells10010132