6533b86efe1ef96bd12cc810
RESEARCH PRODUCT
Cell wall composition and protoplast regeneration in Candida albicans
Rafael SentandreuHortensia RicoDaniel GozalboM V Elorzasubject
macromolecular substancesMicrobiologyMicrobiologyCell wallCell membraneFungal Proteinschemistry.chemical_compoundChitinCell WallCandida albicansmedicineCandida albicansMolecular BiologyGlucansMyceliumGlucanchemistry.chemical_classificationChitin SynthasebiologyProtoplastsfungiGeneral MedicineProtoplastbiology.organism_classificationYeastmedicine.anatomical_structureBiochemistrychemistrydescription
The transition of blastospores to the mycelial phase in Candida albicans was induced after the blastospores were kept at 4°C for several hours and then transferred to a fresh medium prewarmed at 37°C. Glucan was the most abundant polymer in the wall in the two morphogenetic forms but the amount of chitin was higher in the mycelial form than in blastospores. Efficient protoplasting required reducing agents and proteases together with β-glucanases (zymolyase). Protein synthesis in regenerating protoplasts was initiated after about 30 min. Chitin synthetase, initially very low, was incorporated in important amounts into cell membranes mainly in a zymogenic state. After a few hours chitin was the most abundant polymer found in the aberrant wall of the regenerating protoplast.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1983-01-01 |