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RESEARCH PRODUCT

Characterization of two groups of Spodoptera exigua Hübner (Lepidoptera: Noctuidae) C-type lectins and insights into their role in defense against the densovirus JcDV.

Laila GasmiSalvador HerreroAgata K. JakubowskaMarie Helene OgliastroJuan Ferré

subject

0106 biological sciences0301 basic medicineJcDVS. frugiperdaPhysiologyparvovirusesmedia_common.quotation_subjectInsectSpodopteraSpodoptera01 natural sciencesBiochemistrydensovirusLepidoptera genitalia03 medical and health sciencesSpecies SpecificityPhylogeneticsExiguaAnimalsC-type lectinsLectins C-Typeinnate immunityPhylogenymedia_commonGeneticsinsect immunityInnate immune systembiologyfungiDensovirinaeGeneral MedicineSequence Analysis DNAbiology.organism_classificationinfectionbracovirus010602 entomology030104 developmental biologyInsect ScienceLarvaNoctuidaeInsect Proteinsinsect[SDE.BE]Environmental Sciences/Biodiversity and EcologyDensovirusimmunitéS. exiguapathogenagent pathogène

description

Insect innate immunity relies on numerous soluble and membrane-bound receptors, named pattern recognition proteins (PRPs), which enable the insect to recognize pathogen-associated molecular patterns. C-type lectins are among the best-studied PRPs and constitute the most diverse family of animal lectins. Here we have characterized two groups of Spodoptera exigua C-type lectins that differ in their phylogeny, domain architecture, and expression pattern. One group includes C-type lectins with similar characteristics to other lepidopteran lectins, and a second group includes bracoviral-related lectins (bracovirus-like lectins, Se-BLLs) recently acquired by horizontal gene transfer. Subsequently, we have investigated the potential role of some selected lectins in the susceptibility to Junonia coenia densovirus (JcDV). For this purpose, three of the bracoviral-related lectins were expressed, purified, and their effect on the densovirus infection to two different Spodoptera species was assessed. The results showed that Se-BLL3 specifically reduce the mortality of Spodoptera frugiperda larvae caused by JcDV. In contrast, no such effect was observed with S. exigua larvae. In a previous work, we have also shown that Se-BLL2 increased the tolerance of S. exigua larvae to baculovirus infection. Taken together, these results confirm the implication of two different C-type lectins in antiviral response and reflect the biological relevance of the acquisition of bracoviral genes in Spodoptera spp.

10.1002/arch.21432https://pubmed.ncbi.nlm.nih.gov/29164671