6533b86ffe1ef96bd12cd49c

RESEARCH PRODUCT

Vibrio gallaecicus sp. nov. isolated from cultured clams in north-western Spain.

Alicia E. ToranzoJesús L. RomaldeJavier PascualRoxana Beaz-hidalgoAlejandra Doce

subject

DNA BacterialMolecular Sequence DataAquacultureApplied Microbiology and BiotechnologyMicrobiologyDNA RibosomalMicrobiologyBacterial ProteinsSpecies SpecificityPhylogeneticsVibrionaceaeRNA Ribosomal 16SAnimalsEcology Evolution Behavior and SystematicsPhylogenyVibrioPhylogenetic treebiologyNucleic Acid HybridizationGenes rRNASequence Analysis DNARibosomal RNAbiology.organism_classification16S ribosomal RNAVibrioHousekeeping geneBacterial Typing TechniquesBivalviaPhenotypeSpainSpectrometry Mass Matrix-Assisted Laser Desorption-IonizationAmplified fragment length polymorphismPolymorphism Restriction Fragment Length

description

Abstract A group of three motile facultative anaerobic marine bacteria were isolated from cultured Manila clams (Ruditapes philippinarum) in Galicia, north-western Spain. The strains were characterized phenotypically and genotypically. Phylogenetic analysis of the 16S rRNA gene and four housekeeping genes, RNA polymerase α-chain (rpoA), RecA protein (recA), the α-subunit of bacterial ATP synthase (atpA) and the uridine monophosphate (UMP) kinase (pyrH), indicated that these strains were closely related to the Vibrio splendidus clade. The amplified fragment length polymorphism (AFLP) fingerprints, DNA–DNA hybridizations and phylogenies of the housekeeping and 16S rRNA gene sequences showed that the three strains represented a different species from all currently described vibrios. The new species could be differentiated from its nearest neighbours on the basis of several phenotypic features. The three strains are therefore a novel species within the genus Vibrio, for which the name Vibrio gallaecicus is proposed, with the type strain being VB 8.9T(=CECT 7244T=LMG 24045T).

yearjournalcountryeditionlanguage
2009-02-03Systematic and applied microbiology
10.1016/j.syapm.2008.12.002https://pubmed.ncbi.nlm.nih.gov/19186015