6533b86ffe1ef96bd12cdd0f
RESEARCH PRODUCT
Androgen receptors and hormone sensitivity of a human prostatic cancer cell line (PC-3) are modulated by natural beta-interferon.
Fortunata IacopinoGigliola SicaT.h. Van Der KwastA. FattorossiG. Dell’acquaMichele Pavone-macalusoP. Marchettisubject
Malemedicine.medical_specialtymedicine.drug_classUrologyDrug Resistanceurologic and male genital diseasesAntiandrogenchemistry.chemical_compoundInternal medicinemedicineTumor Cells CulturedHumansCell growthCell CycleProstatic NeoplasmsAndrogen AntagonistsDihydrotestosteroneInterferon-betaCell cycleAndrogenImmunohistochemistryFlutamideAndrogen receptorEndocrinologychemistryCell cultureReceptors AndrogenDihydrotestosteroneAndrogensHydroxyflutamideCell Divisionmedicine.drugdescription
Androgen receptors are expressed at a low level in the cell line PC-3, which does not respond to either androgens or antiandrogens. If these cells are exposed to natural beta-interferon (beta-IFN) a reduction in cell growth and an increase in androgen receptors, evaluated by both biochemical and immunocytochemical techniques, occur. This increase seems not to be related to a selective block of PC-3 in any phase of the cell cycle. Pretreatment with beta-IFN determines in PC-3 cells a partial responsiveness to the androgen dihydrotestosterone as reflected by the increase in cell number. Moreover, the antiandrogen hydroxyflutamide shows agonistic properties by increasing the cell number of PC-3 cells pre-exposed to beta-IFN. When the antiandrogen is tested in combination with interferon, it produces a reduction in the beta-IFN-induced inhibition of cell growth. It is not known whether these unexpected effects are due to the increase in androgen receptors or to other mechanisms.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1994-04-01 | Urological research |